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PFKM Rabbit mAb [Z8VW]Cat NO.: A63829

Western blot(SDS PAGE) analysis of extracts from SHSY5Y cells .Using PFKMRabbit mAb [Z8VW] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PFKM,PFKX,PFKAM_HUMAN,ATP-dependent 6-phosphofructokinase, muscle type

UniProtID :P08237

MASS(da) :85,183

MW(kDa) :85 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat,Pig

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PFKM

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SHSY5Y cells 

Function : Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.