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Phospho-FoxM1 (Thr600) Rabbit mAb[0M9B]Cat NO.: A70798

Western blot(SDS PAGE) analysis of extracts from HeLa cells, treated with synchronized in mitosis with Nocodazole (100 ng/ml, 16 hr)..Using Phospho-FoxM1 (Thr600)Rabbit mAb IgG [0M9B] at dilution of 1:1000 incubated at 4℃ over night.

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  • 100ul2122.00库存充足
  • 200ul3198.00库存充足
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Product information

Protein names :FOXM1,FKHL16,HFH11,MPP2,WIN,FOXM1_HUMAN,Forkhead box protein M1

UniProtID :Q08050

MASS(da) :84,283

MW(kDa) : 125

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr600 of human FoxM1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells, treated with synchronized in mitosis with Nocodazole (100 ng/ml, 16 hr).

Function : Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed:19160488, PubMed:20360045). Plays a role in the control of cell proliferation (PubMed:19160488). Also plays a role in DNA break repair, participating in the DNA damage checkpoint response (PubMed:17101782). Promotes transcription of PHB2 (PubMed:33754036)..

Tissue specificity :Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.