P-Acetyl-CoA Clase (S79) Rabbit mAb [C07I]Cat NO.: A72681

Protein names ：ACACA,ACAC,ACC1,ACCA,ACACA_HUMAN,Acetyl-CoA carboxylase 1

UniProtID ：Q13085,O00763

MASS(da) ：265,554

MW(kDa) ：280 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-Acetyl-CoA Clase (Ser79)

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：SH-SY5Y cells treated with Oligomycin (0.5 μM 30 min)

Function ： Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20952656, PubMed:20457939, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20952656, PubMed:20457939, PubMed:29899443)..

Tissue specificity ：Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues,expressed at low level in pulmonary tissue,not detected in the liver.

Subcellular locationi ：Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.