βIII-Tubulin Mouse mAb [6KT2]Cat NO.: A74199

Protein names ：TUBB3,TUBB4,TBB3_HUMAN,Tubulin beta-3 chain

UniProtID ：Q13509

MASS(da) ：50,433

MW(kDa) ： 50KDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse monoclonal IgG

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human βIII-Tubulin.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：293T cells

Function ： Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977)..

Tissue specificity ：Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues..

Subcellular locationi ：Cytoplasm, cytoskeleton. Cell projection, growth cone. Cell projection, lamellipodium. Cell projection, filopodium.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.