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Hsp27 Mouse mAb [1NXR]Cat NO.: A69790

Western blot analysis of extracts from Rat lung tissue lyaste.using Hsp27 Mouse mAb [1NXR] at dilution of 1:1000 incubated at 4℃ over night

  • SizePriceIn stock
  • 100ul2088.00库存充足
  • 200ul3098.00库存充足
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Product information

Protein names :HSPB1,HSP27,HSP28,HSPB1_HUMAN,Heat shock protein beta-1

UniProtID :P04792

MASS(da) :22,783

MW(kDa) :27 kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Hsp27.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Rat lung tissue lyaste

Function : Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742)..

Tissue specificity :Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle..

Subcellular locationi :Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.