DENN Rabbit mAb [4QWO]Cat NO.: A77369

Protein names ：MADD,DENN,IG20,KIAA0358,MADD_HUMAN,MAP kinase-activating death domain protein

UniProtID ：Q8WXG6

MASS(da) ：183,303

MW(kDa) ：220 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of Human DENN.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells lyastes

Function ： Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family (PubMed:20937701, PubMed:18559336). Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms (PubMed:20937701, PubMed:18559336). Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion (By similarity). Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity). Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity). Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity). Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PubMed:32761064). May link TNFRSF1A with MAP kinase activation (PubMed:9115275). May be involved in the regulation of TNFA-induced apoptosis (PubMed:11577081, PubMed:32761064)..

Tissue specificity ：Expressed in testis, ovary, brain and heart (PubMed:8988362). Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon (PubMed:9115275). Expressed in liver (PubMed:9796103)..,[Isoform 1]: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..,[Isoform 2]: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..,[Isoform 3]: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..,[Isoform 4]: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..,[Isoform 5]: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..,[Isoform 6]: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..,[Isoform 7]: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid..

Subcellular locationi ：Cell membrane. Cytoplasm. Cell projection, axon.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.