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Western blot analysis of extracts from Human fetal heart tissue lyaste.using NDUFA8 Rabbit mAb [1048] at dilution of 1:1000 incubated at 4℃ over night
Protein names :NDUFA8,NDUA8_HUMAN,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
UniProtID :P51970
MASS(da) :20,105
MW(kDa) :17 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NDUFA8.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal heart tissue lyaste
Function : Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed:27626371, PubMed:32385911, PubMed:33153867). Complex I functions in the transfer of electrons from NADH to the respiratory chain (PubMed:27626371). The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:27626371)..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein. Mitochondrion intermembrane space. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.