GARS Rabbit mAb [J41H]Cat NO.: A90816

Protein names ：GARS1,GARS,GARS_HUMAN,Glycine--tRNA ligase

UniProtID ：P41250

MASS(da) ：83,166

MW(kDa) ：83KDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Rat,Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of Human GARS.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Mouse brain tissue lyaste

Function ：Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:17544401, PubMed:28675565, PubMed:24898252). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:19710017).

Tissue specificity ：Widely expressed, including in brain and spinal cord..,[Isoform 2]: Expressed in brain, spinal cord, muscle, heart and spleen..,[Isoform 1]: Expressed in brain, spinal cord, muscle, heart, spleen and liver..

Subcellular locationi ：Cytoplasm. Cell projection, axon. Secreted. Secreted, extracellular exosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.