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Smad3 (S423/S425) Rabbit mAb [JUZK]Cat NO.: A11171

Western blot(SDS-PAGE) analysis of extracts from C6 cells lysate treated with PMA/TPA.using Smad3 (S423/S425) Rabbit mAb [JUZK] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 100ul2088.00库存充足
  • 200ul3098.00库存充足
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Product information

Protein names :SMAD3,MADH3,SMAD3_HUMAN,Mothers against decapentaplegic homolog 3

UniProtID :P84022

MASS(da) :48,081

MW(kDa) :55KDa

Form :Rabbit IgG

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around S423/S425 of Human Smad3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :C6 cells lysate treated with PMA/TPA

Function : Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator..

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.