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Tri-Methyl-Histone H3 (Lys4) Rabbit mAb [IW01]Cat NO.: A97326

Western blot(SDS PAGE) analysis of extracts from HeLa acid extract Cells lysate.Using Tri-Methyl-Histone H3 (Lys4) Rabbit mAb [IW01] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul11605 day
  • 100ul21885 day
  • 200ul31885 day
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Product information


UniProtID :Q16695

MASS(da) :15,404

MW(kDa) :17kda

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthetic trimethylated peptide around K4 of human Histone H3

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa acid extract Cells

Function : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subcellular locationi :Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.